Puppies 2013

Puppies 2013 - 24/6/2013

Meet the Parents

Carefully selected for temperament, health and conformation

Aust Ch Tambuzi How Soon Is Now

Sierra

Sierra has a wonderfully happy-go-lucky playful temperament. She is Miss personality plus, playful if not a little high spirited. She was, until the birth of this litter, the ‘baby’ of the house. She insists that all visitors must be meet with a wag of the tail and a kisses. Given the chance, she will also ensure the cleanliness of the ears of all visitors.

HEALTH INFORMATION
Fanconi Syndrome: Normal by Parentage History
Pyruvate Kinase Deficiency: Normal by Parentage History
PRA-BJ1: Carrier
AVA Hip Score: 2:3 Score 5
PennHIP: Pending
PPM: Minor strands of ‘no clinical significance’ - ACES screening at 7 weeks old.
Coloboma: Normal
PRA: Normal
Cataracts: Normal

Homozygosity by DNA 89% (Breed average 82%)

Aust Ch Debrak On The Road Again (AI)

Ringo

A clown, a comedian and a fantastic ambassador for the Basenji. Ringo is a absolute delight to live with. Always happy and a real larrikin. This kennel prides itself on many things.Temperament is one of those. I am thrilled that puppies from Ringo’s first breeding have fabulous temperaments for which Tambuzi is becoming reknowned.

HEALTH INFORMATION
Fanconi Syndrome: Normal
Pyruvate Kinase Deficiency: Normal
PRA-BJ1: Normal
AVA Hip Score: 2:2 Score 4
PennHIP: Pending
PPM: Minor strands of no clinical significance
Coloboma: Normal
PRA: Normal
Cataracts: Normal

Homozygosity by DNA 74% (Breed average 82%)

Puppies 2013

Interested to know about future litters? Enquire below....

Puppy Purchase

Careful consideration to the dog breed suitable for your lifestyle is critical when deciding which breed will make the best companion for your family. Puppy purchasers should be aware that Basenji puppies will normally go on to live 12-16 years. Good nutrition and veterinary care, especially in old age can be expensive. All dog owners have an obligation for responsible dog ownership. Purchasing a puppy from Tambuzi comes with significant benefits:

Puppy Pack - Offered with Puppies 2013

Healthy well adjusted puppy of at least 8 weeks old (puppies will not be homed earlier than 8 weeks)
Puppy will have up-to-date vaccinations to the date or transfer
Puppy that is fully wormed up until the date of transfer
Puppy will be microchipped
Puppy will be registered with Limited Registration with the ANKC (DOGS Victoria). Pedigree papers are supplied
Puppy will be eye screened at 6-7 weeks by an Australian Canine Eye Scheme eye specialist
Copy of the ACES eye screening examination
Puppy will be DNA profiled (DNA fingerprint)
Copy of DNA profile with verified disease and traits (this will outline any molecular DNA tests carried out on the puppy and will list any tests (OFA and other agencies) for which the puppy is verified as being 'normal by parentage history'
Copy of parentage verification (important for 'normal by parentage history' health results)
Copy of parents health certification
Information pack: diet sheet; socialisation; training; raising a puppy; vaccination information; worming information; breed health information; photos of the pup as he/she develops
Toy
Contract of sale: signed by all parties. The contract is designed to protect the puppy in all instances. Certain assurances are outlined in this document. Any special conditions of sale will be noted in the contract. Puppies sold as companions are sold on a de-sexing contract.
Lifetime return: if for whatever reason a puppy is not longer wanted Tambuzi will take the puppy back at any time
Lifetime support: questions, problems and issues are diligently answered. Assistance with any problem no matter how small for the dogs life.

Enquiries

Basenji Puppies

My litter of puppies 2013 was born on the 24th June. This was exactly on time. Sierra's 5 boys (tri colours and red & whites) and 2 girls who are both red & white did tremendously. Mum was incredible with her first litter. But she had assistance from her Mum Halle who produced milk and was happy to lend a hand feeding. Plus Meja, who at 14 didn't produce milk but took up cleaning the pups whilst they were being fed. The three 'mums' would lie in a circle with the pups nice and warm in the center. It was wonderful to see so cooperation and teamwork.

There are many factors for consideration when selecting prospects for the sire (Father) and dam (Mother) of any litter including the health, temperament, conformation and zygosity of the parents. Obviously, we cannot control what nature produces but by testing, research and analysis we can endeavour to reach outcomes to benefit the breed and owners alike. To that end Tambuzi’s primary goal is to produce sound Basenjis, sound in both conformation and temperament. Dog sports such as showing, obedience, agility, tracking etc. can be exciting and entertaining (for Basenji and human). However, I realise that, in reality, most puppies will become faithful companions and will not necessarily take part in such sporting activities.

Deciding on a breeding is done carefully, generally I will not breed until I require a puppy to continue my breeding program and until I have sufficient enquiries to furnish the puppies with the very best homes.

Selection for Health

The advent of affordable DNA testing for health and disease control has made consideration to health, when selecting breeding pairs, much easier to determine. I have an extensive health management program which dictates what is acceptable to breed with and what is not. Nowadays there are many tools available to breeders; DNA testing for disease, DNA analysis of zygosity and various health screening programs. The Basenji is a fairly resilient breed. However, the breed, like most other dog breeds, does have some health challenges. The good news is that with use of a number of health screening 'tools' breeders can eliminate disease. Pyruvate Kinase Deficiency (Haemolytic Anaemia) is one example where testing has virtually eliminated the disease. The very quick reduction in the prospect of Basenjis succumbing to Fanconi Syndrome is a direct result of the DNA test now available.

The DNA results of the parents of Tambuzi's 2013 litter dictate that the puppies will have the following health results:

Normal for Fanconi Syndrome (by parentage history); the puppies will not inherit the mutant gene responsible for the disease and therefore, the disease will not be inherited.
Normal for Pyruvate Kinase Deficiency (by parentage history); the puppies will not inherit the mutant gene responsible for the disease and therefore, the disease will not be inherited.
Normal or Carrier for PRA-BJ1 – Basenji Night Blindness; the puppies might inherit one copy of the mutant gene. Those that inherit one copy of the mutant gene are known as carriers. Carriers do not become clinically affected. Carriers can and if they are of breeding quality should be bred from to maintain genetic diversity.

What is ‘Normal by Parentage History’ ?

Put simply this means that both parents of a puppy have been tested for the disease and the result of each parent is normal. Normal meaning that both parents are free from the mutant gene responsible for the disease, and therefore, the puppies will not become afflicted by or carry the disease. However, puppy buyers should note that in order to legally claim a puppy is Normal by Parentage History (sometimes referred to as Clear by Parentage) several conditions must be in place and it is in the puppy buyers best interest to ensure that the documentation and paperwork are in place:

Both parents must be normal and in the case of Fanconi Syndrome this must be by the ‘direct test’. Normal by the linkage marker test resulting in a probable result (‘probable normal/clear’) cannot be used in a ‘clear by parentage’ scenario. The word probable is indicative of the fact that the linkage marker test does not test for the precise mutant gene responsible for a condition. Linkage marker tests invariably give inaccurate results.
Both parents must be DNA profiled (DNA fingerprint) – the DNA certificates should be available for buyers to review.
The dog in question must be DNA profiled (DNA fingerprint)
The dog in questions’ parentage must be proven by DNA. Research by the American Kennel Club found that (across all breeds) 10% of sires were not the sires of the dog in question noted on pedigree documentation; often a non-witnessed mating with another dog had taken place either before or after the desired mating was witnessed. In the case of Normal by Parentage History, the verification of parentage certificate should be available for puppy buyers to view.

Why is knowing the parentage important to me, my puppy is a companion, I am not going to breed?

In short, accidental matings unbeknown to the owner can and do take place. This means that it is possible for a litter to be sired by a dog other than the one intended by the breeder. This unwitnessed mating might be one that is most undesirable for the health outcomes for the puppies. A female may also be mated by multiple sires and successfully deliver puppies sired from such a multiple mating. DNA profiling of each puppy for parentage is the only certain method to ensure (where as mentioned below, DNA tests are available) that there are no unexpected health issues.

Desirable and Undesirable Matings?
To maintain genetic diversity it is important, especially in a breed with a relatively small gene pool, not to discard carriers of recessive diseases from the breeding pool. A desirable mating is one where the health outcomes for puppies are positive. A carrier can be mated to a normal or even an affected safely, without the offspring having the potential to be afflicted for the disease. However, mating a carrier to a carrier, carrier to affected or affected to affected does have the potential to produce the disease in the puppies and these breeding combinations should be avoided and are therefore undesirable. In the case of recessive disease both parents must carry the disease for it to be produced in the puppies. One can imagine the outcome if that accidental or non witnessed mating was between two carriers. Without DNA profiling each puppy no one would be any the wiser, until that is, a puppy unexpectedly produced the symptoms. Tambuzi provides puppy buyers with proof of parentage - this ensures some certainty and confidence to puppy buyers that (where there are DNA tests available) there will be no unexpected health issues.

AS PART OF THE COMMITMENT TO BASENJIS AND their OWNERS TAMBUZI PROVIDES A COPY OF THE DNA PROFILE OF a PUPPY to owners. A DOCUMENT that verifies PARENTAGE IS ALSO PROVIDED. These documents provide owners the ability to use the on-line parentage verification software at ASAP's website. WHERE DNA TESTS ARE AVAILABLE, PUPPY BUYERS WILL KNOW, IN ADVANCE, THE HEALTH STATUS OF THEIR PUPPY.

Predicted health outcomes for this litter

Fanconi Syndrome - Normal 100%

Fanconi Syndrome - Carrier 0%

Fanconi Syndrome - Affected 0%

Pyruvate Kinase Deficiency - Normal 100%

Pyruvate Kinase Deficiency - Carrier 0%

Pyruvate Kinase Deficiency - Affected 0%

PRA - BJ1 - Normal 50%

PRA - BJ1 - Carrier 50%

PRA - BJ1 - Affected 0%

Puppies from this litter will be normal (clear) for Fanconi Syndrome and Pyruvate Kinase Deficiency. Based on probability 50% of the litter may be normal (clear) of PRA-BJ1 and 50% may carry the disease. However, these percentages are a guide only, 100% may be normal (clear) or 100% may carry the disease. It should be noted that carriers only 'carry' one copy of the mutant gene responsible for this disease. Therefore, they will not actually become afflicted by it.

Genetic Diversity

In January 2012 I began DNA profiling my dogs with the cutting edge technology to provide me with a genetic ‘make-up’ of each dog. This state-of-the-art genetic evaluation is an additional tool I will be using for future breedings. Each dogs DNA is evaluated. A dog has 38 autosomes, 25 of these are evaluated during this process. Each dog is compared to other dogs of the breed in the database. The results of each dog can then be compared to a likely ‘breeding mate’ to assist with;

Genetic diversity – this technology has proven that each puppy's genetic make-up can be quite different from its litter mates. Therefore, allowing for the line-breeding of the most genetically diverse pairs. Research has shown that puppies from closely line-bred litters can be more genetically diverse than those of an out-crossed breeding.
Health – a number of autosomal recessive disorders cluster around chromosome 12, therefore by evaluating the haplotypes on this chromosome and avoiding ‘cross-over’ or duplication of the haplotypes the likelihood of these diseases being passed to progeny is reduced. Tambuzi's 2013 litter has 0% cross over at chromosome 12.
Litter Numbers - Research has shown that average litter numbers will increase by carefully using the results from this technology.

However, this is only one tool and other factors; conformation, phenotype, temperament and other health results all go into the mix when deciding who to mate with whom.

The breed average homozygosity (level of inbreeding) is 82%. A higher (than other 'man-made breeds) in breeding percentage would be expected from a native dog breed like the Basenji.

Ringo has a homozygosity of 74% and Sierra 89%. The predicted homosygosity of the puppies is 83%. The pups overall breeding score is 7 out of 25. The lower the breeding score the less risk the puppy has of inheriting 2 copies of the same haplotype on the same chromosome. Therefore the lower the risk of disease.

Puppies 2013 - 24/6/2013

Meet the Parents

Aust Ch Tambuzi How Soon Is Now

Aust Ch Debrak On The Road Again (AI)

Puppies 2013

Interested to know about future litters? Enquire below....

Puppy Purchase

Puppy Pack - Offered with Puppies 2013

Enquiries

Basenji Puppies

Selection for Health

What is ‘Normal by Parentage History’ ?

Why is knowing the parentage important to me, my puppy is a companion, I am not going to breed?

Predicted health outcomes for this litter

Genetic Diversity

In Combination (Sierra x Ringo) - Breeding Score = 7

Contact

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